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The most likely genomic origin of each transcript was determined by performing blastN searches [ 46] (word size = 32; e-value threshold = 1e-10).
To determine the most likely genomic source of sequences aligning antisense within the 3' UTR clusters, these sequences were extracted and re-aligned to the genome under strict conditions (up to one mismatch, unique alignments only).
We suggest that, in the same vein, the hunt for distal variants causing transcriptional mis-regulation of a disease-associated gene should first be performed in the TAD in which the gene in question lies, as this is the most likely genomic interval to harbor its long-range control elements.
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We believe these findings most likely reflect genomic rearrangements that have occurred since the divergence of these two species.
The differences observed in the complete data set were negligible, most likely because genomic relationships were a small fraction of all relationships.
QTL regions recurrently identified in different pedigrees and environments are most likely robust genomic regions controlling the variation of the traits.
These results indicate that genetic improvement in bTB resistance is possible, most likely through genomic selection methods originally suggested by Meuwissen et al. (2001), rather than relying on individual SNPs, whose frequency in the population as a whole is still unknown.
Analysis of all differentially present genes is of utmost importance, since these genes denote the accessory genome, which most likely represents the genomic repertoire that explains for a large part observed virulence, pathogenicity or transmission differences between clones.
To take the effect of uncertainty in genotype calling on imputation accuracy into account, we considered the possibility of using the genotype probability instead of the most likely genotype for genomic prediction, which is expected to increase the reliability of genomic prediction [ 40].
The BLAST matches were examined based on the alignment score and E-value to identify the most likely matches and genomic location of the corresponding prohormone.
Therefore these contigs most likely do represent genomic hypomethylated regions, especially as all reads which showed high similarity to the plastid sequences of the chloroplast and the mitochondrion were filtered in the initial preprocessing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com