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But the question of what to do with the surplus is proving just as contentious as debate over the shortfall two years ago, and will most likely frame much of the wrangling throughout the fast-paced 140-day regular session.
What follows is a look at his legacy in five areas — politics, education, crime, race and business — that will most likely frame Chicagoans' discussions about the eventful mayoralty of Richard M. Daley.
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This algorithm calculates the coding potential across a sliding window of user-defined size (we used 120) for all six frames and determines the most likely coding frame.
HMM (hidden markov model) protein families from the PFAM [ 65] and TIGR [ 66] databases were assigned to the amino acid translation of the most likely reading frame (identified using an open reading frame prediction tool [ 67]) of each transcript using HMMscan [ 68] under default settings.
Ms. Haley, who said that she would not appoint herself, is most likely to frame her choice around her own re-election efforts.
In all cases selection of an alternative start codon in the most likely reading frame would i) bring the predicted protein sequence length in better agreement with the predicted protein sequence length of the close by orthologs and ii) add a predicted signal peptide feature to the alternative N-terminal end of the predicted CBS 513.88 protein.
The procedure used to identify bZIPs in EST datasets was identical to that used for genomic sequences, except that the estwisedb program of the Wise2 package [107] was included to identify the most likely reading frames and its bZIP domains in a given cluster.
We used custom Perl scripts to capture the best BLAST hit on the forward frame and determine the most likely open reading frame (ORF) for each sequence.
Joint estimation methods [19 21] can evaluate a set of possible f0 hypotheses, consisting of f0 combinations, selecting the most likely at each frame without corrupting the residual as it occurs with iterative cancellation.
According to the highest BLASTX and/or BLASTN hits, the most likely open reading frames were annotated, and their corresponding 3' UTRs were determined through to the polyadenylation signal.
Branch lengths between strain 195/DONNA2 were calculated from single-gene trees of the 387 core protein encoding genes, after excluding 45 trees that did not have strain 195 and DONNA2 as a monophyletic group, most likely resulting from frame-shift mutations.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com