Sentence examples for most intense transition from inspiring English sources
The most intense transition (SRM 1) was used to calculate the LOD, while the second most intense transition (SRM 2) was used to confirm the presence of the compound.
Samarium ion emits mainly in visible region with the most intense transition in the orange-red portion of the spectrum.
Of the monitored MRM transitions (Table S-5, Supporting Information), glycopeptide precursor → 366.1 (Hex–HexNAc) transition, the most intense transition, was used for quantitative comparisons.
Building these methods requires a library of MS/MS spectra for inclusion of the most intense transition pairs (m/ z data for parent ion and sequence fragment ion) for a given peptide.
The selected reaction-monitoring mode (SRM) was performed for the detection of the two most intense transitions at their respective m/z ratios.
Thanks to the innovative configuration and the versatility shown by the dual cell linear ion trap MS used, the most intense and reliable peptide markers were first identified by untargeted survey experiment, and subsequently employed to design an ad hoc multi-target SRM method, based on the most intense transitions recorded for each selected precursor peptide.
The most intense and reliable peptide markers were first identified by untargeted survey experiment using quadrupole-orbitrap high resolution mass spectrometer, and subsequently employed to design a multi-target MRM method, based on the most intense transitions recorded for each selected precursor peptide.
Maximum of two most intense transitions per precursor free from matrix interferences was used.
Peptides chosen for further development were then limited to the four most intense transitions as found in unscheduled runs.
A scheduled SRM method was developed with a retention time window of ±5 min. The four most intense transitions were first subject to collision energy (CE) optimization in ±4 V steps to enhance intensity of detected transitions.
The most intense SRM transition (SRM 1) was selected for quantitation and the second most intense (SRM 2) was used for confirmation.
