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The user has the option of selecting a probe-variation threshold so only the most informative probes are used in the classification process, a decision that can be informed by inspecting an easily generated probe-variation plot.
We obtained similar results using the most informative probes (n = 5444) (Figure S4).
When we clustered the FFPE samples using the 5444 most informative probes the correlation between non-replicate samples changed from 0.91 (using all 21178 probes) to 0.69.
For FFPE and FF paired samples the average Pearson correlation was 0.65 (±0.11) using all probes and 0.70 (±0.10) using only the most informative probes.
For randomly paired samples the Pearson correlation coefficient decreased to values around zero with all probes (r = 0.002±0.01) and with the most informative probes (r = 0.009±0.01).
Although the correlation between replicates was slightly lower when only the most informative probes were used, the correlation between the samples became more specific for sample pairs.
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Our haptic display is therefore a valuable tool to explore the haptic sensation of different neuro-mechanics and to determine the most informative probing motion(s) to be used in order to discriminate them.
Multiple probe sets targeting the same gene were filtered according to their largest variability to emphasize the most informative probe sets.
However, a selection on the most informative probe sets (based on highest variance for each probe set/gene and highest variance between genes) is required.
These results highlight that exon arrays can show a concordance in gene expression compared with FF tissue, but that a selection of the biologically most informative probe sets is required.
The most informative 4 probes were TP63, MYC, CEP3 and CEP6.
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