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Twenty of the most important tick hosts were grouped taxonomically and six group-specific primer sets, targeting short mitochondrial DNA regions, were designed de novo.
Rhipicephalus (Boophilus) microplus is the most important tick vector of A. marginale in tropical and subtropical regions of the world.
Rhipicephalus microplus is the most important tick in tropical and subtropical areas in Mexico and throughout the world, causing great economic losses in livestock production [ 1].
The most important tick vector is the Hyalomma spp., as the virus was isolated from it and its geographic distribution coincides with that of the disease [ 9].
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These novel molecular methods included a multiplex real-time PCR assay to screen for the presence of the most important tick-borne pathogens (piroplasms and anaplasmas), followed by a second species-specific multiplex real-time PCR assay for the identification of Anaplasma-positive samples.
Lyme disease was subsequently recognized as the most important tick-borne disease in North America.
Bovine anaplasmosis is one of the most important tick-borne diseases of ruminants worldwide.
The hard tick Ixodes scapularis and its close relatives, Ixodes pacificus and Ixodes ricinus, are the most important ticks because they transmit the majority of emerging human disease pathogens [ 28, 29].
From a human health perspective, Ixodes scapularis and its close relatives, I. pacificus, I. ricinus and I. persulcatus and I. holocyclus are the most important ticks as they transmit the majority of emerging human disease pathogens.
Amblyomma americanum is considered the most important vector tick for transmission of E. chaffeensis, and deer are an important wildlife reservoir for E. chaffeensis in nature (10, 11).
Rhipicephalus (Boophilus) microplus is considered to be the most economically important tick parasite in the world.
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