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Dim-5 recognizes R8-G12 of the H3 tail with T11 and G12 being the most important specificity determinants.
The Venn diagrams may provide a generally useful tool to highlight the most important specificity determinants for rational redesign.
For instance, position P3 (Y) and P1 (Q) were almost completely invariant in all functional substrates, thereby confirming them as being the most important specificity determinants.
The peptide, ENLYFQG, identical to the protease's natural substrate peptide, emerged as a strong consensus cleavage sequence, and position P3 (tyrosine, Y) and P1 (glutamine, Q) within the substrate peptide were confirmed as being the most important specificity determinants.
This came as a surprise since the glutamate that normally occupies this position is believed to participate in an intricate hydrogen-bonding network in the protease-substrate complex [27], and has been postulated as one of the most important specificity determinants [26], [27].
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However, in this technical review, scalable-based solutions are considered as inefficient due to the fact that poor compression performances and wireless networks are not really studied with their most important specificities at radio link layer like radio frame retransmissions.
However, when the test platform was prioritized for early HIV seroconversion or syphilis, sensitivity was still ranked as most important, but specificity was rated second most important.
Particularly, when the window size L = 11 nt, the algorithm achieved probably the best-balanced performance (the mean values for each metric are ≥ 95.87%), especially in terms of the three most important measures: sensitivity, specificity and accuracy.
Reactive centre loop (RCL) sequences were aligned using the conserved P17 Glu, P14 Thr and P8 Ser/Thr, allowing the reactive centre P1 residue – the most important for inhibitory specificity – to be identified for each serpin.
The above definitions suggest that the region in between amino acid positions 16 30 and 44 58 are the most important for function specificity of genes belonging to different classes and sub-classes.
Undoubtedly, the significance of investigations performed in vitro related to the in vivo situations has to be taken with caution due to the presence of several differences; besides, cell sensitivity is only one factor and is not necessarily the most important in determining specificity of VK1 action.
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