The TTG in the −35 region seem to be the most important nucleotides of the −35 hexamer as these are the most highly conserved [46], which could explain the opposite phenotypes conferred by the C-32A and A-34T mutations.
In mammals, the optimal consensus sequence for initiation is GCCA/GCCAUGG, with a purine at position −3 and a G at position +4 (underlined) being the most important nucleotides [32].
Within the Kozak motif, the most important nucleotides for efficient translation initiation are the R in position -3 and the G in postion +4 relative to the initiation codon [ 25, 26].
Several polymorphisms have been revealed in the IL-6 gene, including one of the most important single-nucleotide polymorphisms (SNPs) in the promoter, the -174G to C substitution, which affect IL-6 levels and are associated with RA, especially with systemic juvenile chronic arthritis.
Among the most important features are biased nucleotide composition favoring adenine-thymine bases and rapid sequence evolution.
The matching is imperfect and the so-called "seed region" (2 8 nucleotide) is most important for target recognition and silencing.
The "core" region of a binding site is defined as a contiguous stretch of nucleotides that is most important for TF-DNA binding.
We find that O/E sites (predicted using MatInspector's less stringent parameters) show statistically significantly higher conservation than the remaining bases (Table 1, Bonferroni-corrected p < 10-63 considering the whole site, or p < 10-129 considering just the core nucleotides that are most important for binding; one-tailed Wilcoxon tests).
The 'seed' region located at miRNA nucleotides 2-8 is the most important sequence for interaction with mRNA targets [ 7].
This indicates that the need to establish nucleotide homology is not the most important obstacle when exploring the phylogenetic structure of the SSU rDNA in planktonic foraminifera.
