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Proliferation, migration and angiogenesis were the biological processes most significantly associated with this signature, and MYC was the most highly networked master regulator.
Disruption of the actin cytoskeleton causes fragmentation of all but the most highly networked mitochondrial tubules, better revealing fenestrated mitochondria that might result from a decrease in the rate of mitochondrial division (Bleazard et al. 1999).
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IPA program gave a score for each signaling network according to the gene expression ratios, in which highest score indicated the most highly regulated network.
One of the most highly rated network filters is OpenDNS.
The most highly crosslinked networks exhibited the maximum volume shrinkage.
For the second most highly significant network, nearly all of the genes represented as green (down-regulated) were found in the GO categories highlighted in Supporting Information, Table S5.
The most highly connected network identified represented a cluster of genes involved in glutamatergic synaptic transmission.
The most highly scored network highlighted the roles for oxidative stress and mitochondrial dysfunction pathways.
The most highly ranked network hub gene, although it is of unknown function, is of great interest.
To identify all relevant genes based on both the fold-change and the network score, the most highly-scored networks for each time point were merged and investigated separately (See Supplementary Figures S1, S2, S3).
We used the jActiveModules (version 2.2) plugin [29], [40] to identify the 20 most highly dioxin-altered network neighborhoods (modules) for each timepoint (days 1 5).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com