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Sam50 was most highly detected within the hydrogenosomal fractions.
As their expression was most highly detected in a population of parasites (either due to higher levels of expression in selected iRBCs or expression in a higher number of iRBCs), these will henceforth be referred to as "dominant cirs".
Moreover, of the 100 proteins most highly detected by shotgun proteomics (de Groot et al. 2009), 27 are produced from a leaderless mRNA and 37 from a leadered mRNA (supplementary table S7, Supplementary Material online).
All nine of the proteins are represented in a whole community proteomic dataset reported previously [ 26], and three are among the most highly detected proteins of this organism in that dataset.
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CDCP1 was the most highly expressed marker detected on our cell line panel comprising 79.1 ± 13.6% of positive cells.
Genes encoding for ribosomal proteins, keratin family members and keratin associated proteins were among the most highly expressed genes detected in sheep skin.
Among the most highly recurrent CNAs detected were loss of regions of CFA 2, CFA 11, CFA 16, CFA 22 and CFA 31, all of which were highly frequent 50-866%) in both breeds.
ATF3, IL-24 and IL-6 were among the most highly induced genes detected in the array analysis (Table 1) and were confirmed to be up-regulated by RT-PCR analysis in all MM cell lines tested.
Not surprisingly, the most highly expressed genes detected in total testis and involved in spermatogenesis (protamine 1, 2, 3 and transition protein 2) formed one of the tight clusters on chromosome 16.
For microvessel quantification, the three most highly vascularised areas detected by CD34 immunostaining were initially selected under the × 40 field, and a × 200 field (0.785 mm per field) was used to count vessels in each of these areas.
We observed that a K+ channel subunit, annexins, porins, secretory pathway components, β-amylase, β-galactosidase and pectin and galactan biosynthetic enzymes were among the most highly enriched proteins detected in developing flax fibres, with many of these proteins showing electrophoretic patterns consistent with post-translational modifications.
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