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(c) Some tubules showed marked degeneration and absence of most germ cells; the rest showed no mature sperms.
c Some tubules showed marked degeneration and absence of most germ cells (black arrows); the rest showed no mature sperms.
Despite entering meiosis properly, most germ cells disappear around the time of birth.
As a consequence, most germ cells from these animals assume a zygotene/early pachytene-like state.
After sex determination, most germ cells enter mitotic arrest [ 20, 21].
Indeed, in most germ cells, switching off of OCT4 is complete by as early as e17.5 (Ferrara et al., 2006).
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As stated by ICH S2 (R1) [6], "most germ cell mutagens are likely to be detected as genotoxic in somatic cell tests so that negative results of in vivo somatic cell genotoxicity tests generally indicate the absence of germ cell effects".
A critical protein involved in the generation of most germ cell piRNA species is Drosophila Spindle-E (Malone et al. 2009).
Finally, our analysis of single cells indicated that in addition to expressing a greater number of germ cell markers within the same cell, single- Oct4-GFP+ cells from day 5 EBs contained significantly elevated levels of most germ cell transcripts analyzed compared with undifferentiated Oct4-GFP+ ESCs (Supplementary Material, Fig. S4).
In gld-1 ; puf-8 hermaphrodite germlines, all proximal-most germ cells became positive for HIM-3 at approximately 36 hr after hatching (mid L3 larva).
In fed controls, incubation at the restrictive temperature caused all germ cells to enter the meiotic cell cycle: Chromosomes adopted the 'crescent' shape, and GLD-1 levels in the distal-most germ cells rose.
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