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The effect of NANOG overexpression on cell survival and self-renewal is also in accordance with the observation that chromosome 12, where the NANOG gene is located, is the most frequently gained chromosome in culture adapted hPSCs (Baker et al., 2007) and during hiPSC generation (Taapken et al., 2011).
The most frequently gained region (>40%) was 8q12.1 24.3, and contained 461 genes.
As previously reported [37], [44], the three most frequently gained regions were on 1q, 8q and 17q chromosomal arms, whereas the regions frequently lost were on 8p, 11q and 16q.
The most frequently gained region in this sample set is chromosome 8q24.
PTK2 (FAK) is the most frequently gained gene overall and PTK2B (PYK) is the most frequently lost kinase.
In addition, the p-arm of this chromosome was the most frequently gained region as indicated by CGH.
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In addition, 12 tumors showed gross alterations of entire or almost entire chromosomes most frequently gain of 5 and loss of chromosome 11.
Plasmid DNA carrying the therapeutic gene most frequently gains access to the nucleus during mitosis, when nuclear envelope rupture occurs [ 4, 5].
In most other countries, however, public health teaching and research has extended regionally within universities which have frequently gained equivalence with the original national school.
Similarly, the gain of 7p is among the most frequently found gains in astrocytomas [ 44].
The most frequently observed gains (> 30% of cases) in the primary tumors were found at 1q, 8q, 16p, 17, and 20, and the most common losses were at 1p, 8p, 11q, 13, 16q, 17p, and 22.
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