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The next most frequent substitution event observed was 2 consecutive base substitutions.
FAP results from mutation of the transthyretin gene, with Val30Met being the most frequent substitution.
The most frequent substitution was AUN (Ile) to GUN (Val) (62 sites), and the next was ACN (Thr) to GCN (Ala) (40 sites).
The two most frequent substitution patterns in SLS were "knee medial to the supporting foot" and "displacement of trunk" (both present in 9/16 participants).
Further, Gly to Arg, Arg to His, and Arg to Gln were the three most frequent substitution mutations in aaRSs (Tables 1 and 4).
AG, CT, GA and TC transitions were found to be the most frequent substitution types (see Additional file 1), which is reflected by a mean transversion/transition ratio (Tv/Ts) of 0.62.
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The aforementioned positions include indeed the most frequent substitutions associated with ESBL-production phenotype (Cohen Stuart et al. 2010).
The most frequent substitutions are C-to-T (26.6%) and G-to-A (25.4%; Figure 4 small RNA sequencing data (Ebhardt et al.2009), which is similar to the results obtained by using the rice and Arabidopsis.
However for the misincorporations originally derived from G and C nucleotides, the most frequent substitutions were type 2 (C→T/G→A) transitions.
Thus, a few C ↔ T transitions, the most frequent substitutions, are enough to transform cryptic simplicity sequences into tandemly repeated DNA.
We focused our analysis on the three most frequent substitutions (connected to its complementary substitutions): the transversion G→T (complementary to C→A) and the two transitions A→G (complementary to T→C) and C→T (complementary to G→A).
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