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Peptides for a given gene product (Env, Gag, Pol) were pooled according to frequency, with pool one containing the most frequent peptides for a given protein.
We rank-ordered peptides based on their frequency in the database calling 'variant 1' the peptides corresponding to the most common epitopic variant (after the consensus) found in circulating sequences, 'variant 2' corresponds to the second most frequent peptides, and so on.
Here we summarize selected findings about the most interesting patterns among the 100 most frequent peptides for each kingdom.
The most frequent peptides were from VGF nerve growth factor inducible (VGF) (5 peptides) and myelin basic protein (MBP) (4 peptides).
The SCG2 prohormone provided the highest number of peptides only detected at daytime (12 peptides), and the two most frequent peptides were SCG2[184 201] (64 spectral counts in 5 samples) and SCG2[571 585] (34 spectral counts in 3 samples).
However, Tachyon achieves a substantial speed advantage by reducing the searched sequence space as a result of associating each entry in the database (e.g. NR) only with representative oligopeptides which are defined as the most frequent peptides of a defined length within the indexed database, excluding low-complexity regions [SEG 12 2.2 2.5 settings (Wootton and Federhen, 1996)].
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Moreover a permanent exclusion list of the most frequent peptide contaminants (keratins and trypsin peptides) was included in the acquisition method in order to focus the analyses on significant data.
The most frequent discontinuous peptide has coverage of 754 strains, while the second most frequent peptide covers 320 strains.
The most frequent peptide pattern in bacterial ORPs from the genomic set, HYNWH (216 occurrences, 9 species), matches 205 copies of transposase from Bordetella pertussis Tohoma I. Similarly, 184 of 190 occurrences of the second most frequent peptide pattern, IMTWM, come from transposase copies from only one species, in this case Mycobacterium ulcerans Agy99.
At position 2, Pro was most frequent (8/12 peptides) with Ala present in the remaining peptides.
Viewed by ranked frequencies, the top 10 most frequent discontinuous peptides covered more than 50% strains in the dataset, and top 25 discontinuous peptides covered nearly 80% of the total strain population.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com