The most frequent cluster (#1) resembled hAQP10 monomers (A D) and was dominant for the monoprotonated simulation.
The most frequent cluster configuration is made of two homoeologous gene copies per genome (one per A and B subgenomes), which occurs in 35% of all unigene groups.
Shown in gray is mean ± STD of the most frequent cluster in 100 randomly shuffled cluster assignments.
The most prevalent pattern in females is ADS and pain (66.4%), while the most frequent cluster in males is cardiovascular/metabolic disorders (79.8%).
Shown is the probability of the most frequent cluster, plotted as a function of stimulus id (purple; stimuli sorted by reliability), for the clustered response; that is, the probability of the most frequent cluster evoked by each stimulus (same as Figure 4A ).
In purple, we plot the reliability of the clustered response, that is, the probability of observing a response from the most frequent cluster for each stimulus (clustering matrix presented in Figure 3 ).
In black is the reliability of the clustered response, that is, the probability of the most frequent cluster evoked by each stimulus, where responses were clustered by similarity derived from a conditionally independent model.
Haplogroup H was the most frequent cluster in European (45%) and Near East (25%) populations [ 16] but only accounted for 13% of Saudi lineages, comparable to the frequency in Bedouin and Yemeni.
Variants grouped into the categories '1-2 bp diff' (IIIa) and '>2 bp diff' (IIIb) which were present in both amplicon replicates but less frequent than any annotated artefact were labelled as 'unclassified variants', since they are not amongst the most frequent clusters but are unlikely to be artefacts if they appear in both replicates in at least two copies each (i.e. in clusters).
This allele was present in four individuals always as the most frequent one (Cluster1) (Additional file 2: Table S2), and the ratio in frequency compared to the second most frequent cluster (Cluster2) ranged in average from 1.9 to 3.9 fold for these individuals, reinforcing the hypothesis that this allele is always present in duplicate.
