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Clone A was by far the most frequent clone and found in 65.9% of all studied strains.
The second most frequent clone among carriers and patients was NmX, ST-181 P1.5-1,10-1;F1-31.
ST 70 is the most frequent clone isolated in Thailand and has also been identified previously in Laos and Hong Kong.
This can be seen by quantifying the rank of the most frequent clone in each population at the termination of the simulation.
The most frequent clone contained a region adjacent to the RNF166 gene, which was subsequently screened for putative TREs by in silico analysis.
However, the second most frequent clone of this sample (0.61% frequency) appeared to be the unmutated Sμ region, implying that either these five mutations accumulated during disease progression or PCR-contamination with germline DNA occurred.
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Two TCCs generated from the CSF and present in L-III (TCC13.1-13 and TCC21-1) were also found in L-I although not among the 25 most frequent clones, but ranking lower regarding frequency (Table 4).
The most frequent clones sequenced had BLASTx hits to: auxin-repressed protein-like protein (115 reads), Arah8 allergen (69 reads), type 2 metallothionein (60 reads), PR10 protein (56 reads) and cytokinin oxidase-like protein (44 reads) (Table 2).
B corresponds to the probability π1 of the most frequent species (clone) [ 48].
As a first screening, wild-type IgG1-Fc (Fc-wt), the parental clone H10-03-6, as well as the 10 most frequent Fcab clones were displayed on the surface of yeast again and analyzed individually for binding to Her2-ECD (3 nM) at pH 6.0 and 7.4.
Notably, the most frequent epidemic clones associated to multidrug resistance in humans, also called 'high-risk clones', i.e. ST111, ST235 and ST175, were not recovered in our collection [ 8].
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