One of the most frequent pitfalls of PCR assays is the contamination of samples, which can occur any time during or after collection of the samples, including during their use in the laboratory.
The most frequent cross-reactions in immunodiagnostic assays for cystic echinococcosis are due to E. multilocularis and T. Solium (13).
In the current study, we compared allele-specific qPCR assays for the most frequent activating mutations in EGFR, KRAS, BRAF and PIK3CA in tumor-positive fine needle cytological aspirates against histological material of primary tumors.
The majority of published assays targets the most frequent IDH1 R132 mutations but they are not designed to detect other rare ones including those within that same codon 132, the IDH1 R100 mutations or the IDH2 mutations.
Samples were assayed for the seven most frequent mutations reported in KRAS codons 12 and 13 (Gly12Ala, Gly12Val, Gly12Ser, Gly12Arg, Gly12Cys, Gly12Cys, and Gly13Asp), accounting for approximately 97% of all KRAS mutations observed in CRC.
Presented results demonstrate, that when using our assay we are able to detect the most frequent sources of aneuploidy in mammalian oocytes – failure to disjoin the whole bivalent or premature segregation of sister chromatids.
Another study using a qRT-PCR assay reported miRNA signatures that correlate with the most frequent cytogenetic alterations (t(15 17), t(8 21), inv(16), 11q23 translocations) in 100 AML samples.
In the present work we developed a simple colorimetric assay using unmodified GNPs to detect 12 of the most frequent point mutations in exon 5, 7, and 8 of human p53 gene.
In order to solve this problem, a multiplex real time assay was designed for simultaneous genotyping and quantification of the 18 most frequent cancer related HR-HPV types and 3 LR-HPV types of HPV-6, −11 and −81.
In an assay on 379 HBOC Slovenian families, c.181T>G, c.1687C>T, and c.844_850dupTCATTAC were the most frequent BRCA1 mutations while c.7806-2A>G splicing alteration which was found in 13 families was the most BRCA2 one [ 86].
