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Using this approach we could determine that alignments where the Arabidopsis lyrata sequences lack a start codon and contain no indels or internal stop codons are the most frequent alignment category representing 53.66% of cases.
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Several investigations analyzing implant alignment in TKA performed with extramedullary systems have found that varus cut is the most frequent error in alignment of the tibial component (Dennis et al. 1993, Teter et al. 1995, Reed et al. 2002).
Consensus nucleotide (or simply consensus) is the most frequent nucleotide in an alignment column (position).
A common remedy is the selection of the most frequent nucleotide at each alignment position to derive the consensus sequence.
The quality score is calculated for each column in the multiple alignment by summing, for all mutations, the ratio of the two BLOSUM62 scores for a mutation pair and each residue's conserved BLOSUM62 score (which is higher); (iii) alignment consensus annotation is composed by the most frequent residue per column of the alignment, hence it represents the percentage frequency of these amino acids.
In the chosen alignment a valine is the most frequent residue in the H5 position and in the alternative alignment threonine would be in the H5 position.
Nineteen positions wherein the CaMdr1p residue matched with the most frequent amino acid at a particular alignment position of DHA1 members were subjected to site-directed mutagenesis and were replaced with either alanine or leucine.
Nineteen out of the thirty highest CRES alignment positions had the most frequent amino acid matching with the CaMdr1p residue.
This overestimation can be explained using Figure 6C, which shows the support (posterior frequency) for the two most frequent topologies for each segment along the alignment.
Here, the quality score of an alignment column is inversely proportional to the average cost of all pairs of mutations in terms of BLOSUM 62 substitution scores and the consensus reflects the fraction of the most frequent residue for each column of the alignment.
Finally, we assigned to each transcript the most frequent gene symbol from the corresponding BLAST alignments to multiple species.
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