The two alternate first exons are conserved throughout all Drosophila species, except D. mojavensis, where the gene, except the most distal first exon is duplicated.
The average size of novel 5′ exons was 129 bp and 135 bp for ovaries and testes, respectively, strongly suggesting that our nebulization strategy (which generated cDNA fragments of, on average, 700 bp) would allow capture of most novel first exons.
The region sequenced in the association panel spanned most of the first exon, including this amino acid substitution (Figure 4B).
In this construct, most of the first exon and the intron of the target genes were replaced by a kanamycin selection cassette (nptII).
Primers for FLC were designed based on A. thaliana genome sequence, and a 5.8-kb fragment encompassing most of the first exon through the 3′ UTR was sequenced.
Genetic variation of eotaxin gene, SCYA11, has been found to contribute to CAD susceptibility, most notably the first exon (67 G/A) polymorphism leading into amino acid change Ala/Thr in site 23, where secreted eotaxin is cleaved off from the signalling peptide [ 23].
These were targeted against the most conserved second exon of HIV-1 TAT/Rev/Env region.
The P1 promoter of TP53 is approximately 1.5 kb upstream to the TP53 transcription start site (TSS), which is composed of a 1088 nucleotide-long non-coding region and most of the WRAP53 first exon.
The 5' UTR, the first exon, and most of the first intron were sequenced in the association panel (Figure 5B).
The first block contains the first exon and most of the first intron, whereas the second LD block contains the remainder of the GHRHR gene.
