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The reason for each field sample submission is often not known nor routinely recorded at the laboratory; however, based on broad summary data from the laboratory, most field samples submitted were from test positive cattle.
However, the test appears useful since it excluded the presence of BSE for most field samples, thus restricting the use of more specific but time-consuming methods, like experimental transmission in mice, to a small number of isolates.
NP carried out most field samples and did the molecular genetic analyses, a first analysis of the genetic data and drafted the manuscript for his Ph.D. MS assisted in field sampling and analysis.
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Extensive sampling is typically required, but in most cases, field sampling is prohibitively expensive and limited by terrain.
The yield of DNA from sediment extraction was low, and therefore nested PCR was necessary for accurate species-specific detection of the three species in most of the field samples.
However, in order to systematically explore relationships and breeding patterns from wild specimens using whole genome sequencing data, high quality DNA is required and in most field studies, non-invasive samples such as feces or hair are used, and the amount of DNA extracted from such samples precludes the application of this methodology to conservation studies.
In this study, inoculation of Pinus radiata with Phytophthora pluvialis resulted in the reproduction of characteristic needle lesions consistent with those from which the pathogen was most consistently isolated from field samples expressing the disease.
This delay fits well with most field conditions, provided the samples must not be sent by post.
Samples from animals exhibiting even subtle signs of BSE are most likely to behave as field samples used in validation exercises used for test approval.This study elucidates the relative performance of these tests solely on experimentally generated C-Type BSE, serially diluted in known BSE-negative tissue homogenate, and not necessarily on C-type BSE occurring by natural means.
The absence of (Z -3-hexenal in our field samples is most probably associated with the unstable nature of this compound.
ZY participated in most of the field sample collecting.
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