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The most extensive intestinal hyperproliferation and adenoma formation resulted from Cre-mediated excision of exon 3 (encoding amino acids 5-80) of the endogenous ctnnb1 gene throughout the intestinal mucosa, thereby deleting all the regulatory serine and threonine residues that control the turnover of the β-catenin protein (Harada et al., 1999; Leedham et al., 2013).
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