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Pinch Analysis (PA) has been one of the most established methods since the 1970's for the design of a maximum heat recovery network.
However, most established methods focus on missing continuous data (1, 2).
For such a short-term projection (3 years), most established methods will provide very similar results.
Most established methods of directed differentiation result in heterogeneous populations of differentiated cells.
A survey and comparisons of the most established methods for the numerical analysis of discrete-state Markov processes are given by Stewart [ 19].
One of the most established methods to investigate automatic language processing with MEG or EEG is the passive oddball paradigm in which the mismatch negativity (MMN) is elicited.
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The most well established methods for computationally predicting RNA structures focus on calculating the secondary structure partition function for an RNA sequence.
Although considerable research has been devoted to finding optimal extraction protocols, most of the established methods require extensive sample handling.
In the absence of a gold standard, the most widely established method to investigate validity is the correlation approach, which allows to assess dissimilarities and complementarities between instruments [ 2, 22].
Here we review the current stage of FRET methods development with the main focus on its applications in protein studies in biological systems, by summarizing the basic components of FRET techniques, most established quantification methods, as well as potential pitfalls, illustrated by example applications.
When assessing the performance and validity of a new technology, it is useful to compare the workflows, costs and, most importantly, the results to established methods.
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