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Most essential genes were found distributed in amino acid metabolism, lipid metabolism, and nucleotide metabolism in case of MG medium.
As expected, most essential genes have homologs in Saccharomyces cerevisiae and Schizosaccharomyces pombe, and the majority of those are also essential.
It is likely therefore that the most essential genes should show a higher degree of dosage compensation in female birds.
The aggregate of genes in clusters include most essential genes involved in translation and transcription, and some photosynthetic genes.
Most pTSS were located on the large replicon (Table 1), which is to be expected since the large replicon harbours most essential genes [ 11].
This suggested that these multi-clade NFn families, which might have emerged in the early common ancestors of Nematoda and remained to be conserved in many nematode species since then, could be the most essential genes required for nematode survival.
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As IKB contains only the most essential immunome genes and does not necessarily contain full pathways, it was supplemented with proteins for key immune system pathways derived from the KEGG Pathway database [ 16] (Table 1).
This cut-off was chosen empirically to minimize the number of false positive genes that were considered unlikely to be essential based on their annotation or prior experimental evidence, whilst retaining most known essential genes.
The Keio collection of single and double gene deletions in Escherichia coli is considered the most comprehensive essential gene study to date [ 43, 69].
Global transcriptome analysis using RNA-Seq indicated that most of the essential genes (92%) are, in fact, transcribed in B. fragilis 638R including most of those coding for hypothetical proteins.
Most of the essential genes were identified in photosynthetic pathways such as photorespiratory cycle (10 genes) and Calvin cycle (7 genes), indicating the rigidity of CO2 fixing mechanism in plants.
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