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However, most enzymatic steps are known.
In addition to the finding that most enzymatic steps were represented by Citrus homologs, a preliminary estimate of gene duplication based on the occurrence of paralogous sequences was also provided.
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Since most of the enzymatic steps are "add only" reactions, there is no material loss due to purification steps and the protocol can be easily automated and performed in 96-well plates.
An automated strategy is also feasible for other massive sequencing library preparation protocols since DNA sample concentrations steps can be replaced by the CA-purification, and most enzymatic reactions are well suited for automation in a robotic workstation.
The GABA-shunt is composed of three enzymatic steps (Figure 1A), and Arabidopsis knockout mutants were isolated for most corresponding genes (with the exception of GAD3, GAD4 and GAD5).
Though the enzymatic steps for the catabolism of l-arabinose were described in the 1960s, most of the genes encoding the enzymes of this five-step pathway were only characterized recently.
Given the number of enzymatic steps required for the addition of sugar moieties, glycosylation is thought to be the most complex PTM, and thus presents particular analytical challenges (Table 1).
Grey arrows indicate enzymatic steps in the lignin biosynthetic pathway.
From quinic acid, it is converted to shikimate by three enzymatic steps using microbial biotransformation [19].
Additional enzymatic steps increase fructose levels.
Unsaturated and odd-chain fatty acids require additional enzymatic steps for degradation.
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