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Response to virus was identified as the most enriched term (15 fold enrichment) in lung comparison, confirming that genes related to virus infection were regulated by miRNAs.
Functional enrichment analysis revealed that the annotation ER-unfolded protein response was the most enriched term after NGF withdrawal suggesting that an ER stress response occurs in sympathetic neurons deprived of NGF.
In enriched GO terms, oxidoreductase activity was the most enriched term.
Among selected MF terms analyzed, 'GPCR binding' was the most enriched term in the immune, respiratory and neurological disease portals.
Strikingly, the most enriched terms were all involved in RNA processing, with mRNA export as the most enriched term (Fig. 3d).
Of note, we found that the most enriched term in 'biological process' category was the regulation of transcription, in which Egr2, Klf4, Klf6, and Rybp was involved.
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The most enriched terms of molecular function were binding, such as nucleic acid binding (GO 0003676), iron ion binding (GO 0005506), and zinc ion binding, (GO 0008270).
The most enriched terms of cellular component were membranes, such as chloroplast outer membranes (GO 0009707), cytoplasmic membrane-bounded vesicles (GO 0016023), and plasma membranes (GO 0005886).
The most enriched terms of biological process ontology were metabolic processes, such as carbohydrate metabolic processes (GO: 0005975), DNA metabolic processes (GO 0006259), cellular macromolecule metabolic processes (GO 0044260), and nucleic acid metabolic processes (GO 0090304).
Concerning genes associated with H3K27me3 only, the most enriched terms in PC3 cells were involved in developmental functions (Figure 4D), while this was not the case in EP156T cells (Figure 4E), suggesting that more repressed genes in PC3 cells were developmental genes.
Statistical function annotation generated additional GO terms, and the most enriched terms might reflect potential lncRNA functions.
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