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Using CD-HIT-EST [ 30], the top 1000 most enriched sequences from each round were clustered by sequence homology to determine possible emergent motifs.
We then chose the 235 most enriched sequences from the HTS data based on copy number, and synthesized these on an Agilent custom DNA aptamer array.
The second and fourth most enriched sequences, however, had consensuses containing the basic 5 bp heat shock element (HSE) motif, nGAAn (nTTCn in reverse complement) and overlapping with the full triple repeat HSE, nGAAnnTTCnnGAAn.
Several GR-like split 8-mers are enriched in GR peaks and the 5 most enriched sequences are 1-bp variants of the canonical GR motif.
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Sequence A3 is the third most enriched sequence in R5.
Sequence B10 is the second most enriched sequence by R4.
Sequence A1 was the most enriched sequence in R5, and it scores moderately well in sequence-based clustering.
ACTTGA appears in the seed position of 3 of the 16 confirmed siRNAs, and was the second most enriched seed sequenced identified by GSEA.
For example, CCGGA CGT is the second most enriched SNP sequence in GABPα+CREB1 peaks in A549 cells, and it is also the most enhanced SNP observed in our in vitro PBM experiments.
As expected, motifs are most enriched in DHS sequences.
Among others, we detected GC sequences and the Sp1 motif, as expected for a TIEG family member, but in addition, one of the most enriched motifs comprised GAGA-binding sequences (Fig 2A).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com