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For each such CPIM, we recomputed the most enriched function.
For outcome-related DEGs, the most enriched function terms in USC group are nucleic metabolism and mRNA transcription, while the most enriched function terms in EAC group are developmental process.
The most enriched function terms for the 689 DEGs specific to USC vs. EAC-G1 are cell cycle, mitosis and amino acid metabolism.
For the 374 DEGs specific to USC vs. EAC-G3, the most enriched function terms are signal transduction, cell communication and oncogenesis.
For stage-related DEGs, the most enriched function terms in USC group are nucleic metabolism, protein traffic and cell cycle, while the most enriched function terms in EAC group are cell structure and mobility, amino acid metabolism and fatty acid metabolism.
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The p-values relative to the most enriched functions appeared highly significant (α = 0.05, Fisher's exact test).
However, after 13 17 h of myxothiazol treatment, the most enriched functions corresponded to the p53 pathway (FDR 1.23E-06).
At the level of the genus Eimeria, DNA and protein binding were among the most enriched functions in novel genes.
The most enriched functions of the up-regulated genes are cell death, cellular development, cellular growth and proliferation, lipid metabolism, and small molecule biochemistry.
Gene Ontology analysis revealed that among the most enriched functions in the group of genes whose regulation by estradiol was lost in both ERβ + cells there were, as expected, DNA Replication, Recombination and Repair, as well as Cell Cycle and Cell Morphology (data not shown).
The most enriched functional group contains genes primarily related to immune function and defense responses.
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