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DAVID only reports significantly enriched clusters; most enriched clusters have the highest score and lowest p-values.
There is a high degree of overlap between the top ten most enriched clusters (Tables S4, S5, S6).
After clustering, the tool reports the most enriched clusters at the level of stringency selected.
From the different FATs that were identified, similar broad functions for the most enriched clusters of both subsets could be identified.
The most enriched clusters of human-specific PE genes are signal/glycoprotein and immunoglobulin, and humans possess a deeply invasive human hemochorial placenta that comes into direct contact with maternal immune cells.
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The most enriched cluster upon Etoposide treatment is the p53-signaling pathway, followed by the functional categories of nuclear envelope and cell cycle regulation (Table 4).
Supporting this notion the analysis of the most enriched clustering categories from CH-dmDys up-regulated genes list revealed an increase in transcript levels of genes related to protein metabolism, biosynthesis, and apoptosis.
Protein localization is the most enriched cluster across all three regions, while vesicle mediated transport and phosphate metabolic processes are within the top five clusters and proteolysis and regulation of GTPase activity are within the top seven for all three tissue samples.
Blue copper protein was the most significant expressed gene that included in cluster 1, which was the most enriched cluster.
One cluster of 14 genes, comprising the third most enriched cluster of the 10 50 RPM piRNA group, was identified to represent functions related to development, gamete generation, and sexual reproduction (Fig. 8).
The most enriched gene clusters that came up in all analyses were related to mitochondria and lipid metabolism.
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