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"Endoplasmic reticulum (ER)" is the most enriched cellular component in this gene cluster.
The most enriched cellular compartment was the 'chloroplast' , and multiple compartments such as 'stroma' and 'envelope' , but not 'thylakoid' , were overrepresented.
Membrane/Insoluble fraction is the most enriched cellular component in cluster 1. Cluster 2 is the smallest cluster among the three and is enriched for "lipid metabolism and lipid transport".
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The most-enriched cellular components in PAR were of cytosolic origin, particularly components associated with cytoskeleton and intracellular non-membrane-bound organelles.
The most enriched GO cellular component (CC) term was "GO: 0001533 cornified envelope".
The most enriched GO cellular component categories were mitochondrial-related, for downregulated genes, and extracellular matrix (ECM -related, for uprECM -relatedes.
The most enriched GO cellular component categories among the protein coding genes are related to ribosomal activity, with an enrichment fold larger than two (cytosolic small ribosomal subunit, 3.43; cytosolic ribosome, 3.2; small ribosomal subunit, 2.43; ribosomal subunit, 2.06).
The most enriched terms of cellular component were membranes, such as chloroplast outer membranes (GO 0009707), cytoplasmic membrane-bounded vesicles (GO 0016023), and plasma membranes (GO 0005886).
Table 1(b) and (c) shows the top three most enriched molecular and cellular functions and top three biological functions as revealed by the IPA Tool.
One of the most enriched categories of cellular components from our list of 270 Dhh1-associated proteins was a set of ribosomal proteins (structural constituent of ribosome, 81 total; hypergeometric p-value = 1 × 10−44).
As for female-biased DEGs, the most enriched GO-terms at cellular component level was mitochondrion and ribosome, the most enriched GO-terms associated with molecular function were aminoacyl-tRNA ligase activity, and the most enriched biology process were translation, cellular biosynthetic process and organic substance biosynthetic process (Table 4).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com