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Within the molecular functions category, genes encoding binding proteins (41.68%) and proteins related to catalytic activity (43.92%) were the most enriched (Additional file 8: Figure S4).
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For the cellular components, GO terms related to extracellular region and plasma membrane were the most enriched (see Additional file 1: Figure S4), suggesting that most of the DEGs were active in these two cellular compartments.
Among the significantly overrepresented motifs of size 4, 5 and 6, the bifan and the feedforward motifs were the top most enriched motifs detected (Additional file 2).
Oxidoreductase activity was the most enriched GO term (Additional file 5), whereas oxidative stress during microsporogenesis was thought to induce a premature abortion of tapetal cells due to programmed cell death (PCD) in CMS sunflower [ 49] and rice [ 50].
After 1 h of exposure to 32°C the imbibed seed has limited response, and genes important for the 'response to high temperature' and 'protein folding' are among the most enriched in early HSRs (Additional file 6).
Analysis of InterPro in these 4727 orthologous genes revealed the most gene-enriched domain and family were peptidase (Additional file 1: Table S13), while analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway revealed that genes were most enriched in metabolic pathways (Additional file 1: Table S14), both indicating functions central to mosquito biology, such as feeding behavior.
The most enriched GO terms shown in Additional file 7 were ranked by the p-values calculated by the "weight" algorithm.
(XLSX 70 kb) Additional file 8: The most enriched pathways that were identified for the target genes.
This observation is consistent with results of the pathway analysis showing that interferon signaling was the most enriched canonical pathway in the overall set (Additional file 10).
Table S2 in Additional File 1 lists the most enriched functional categories for each module.
Statistical function annotation generated additional GO terms, and the most enriched terms might reflect potential lncRNA functions.
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