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The generated pool of DNA templates is analyzed in a cell-free expression screen and the most efficient template is selected for further preparative scale protein production.
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UV-induced cross-linking between RT and the DNA template strand was most efficient when a bromodeoxyuridine residue was placed in the +2 position (the first template position downstream from the incoming dNTP).
This is of note, as Rad51-mediated D-loop formation is most efficient when acting on negatively supercoiled templates.
Formation of the 12-ring is most efficient when using a stoichiometric amount of template (l -P4 C8/T6=1.55).
(a) UV-induced cross-linking between RT and the DNA template strand in the +1 dNTPRTP/T complex was most efficient when BrdU was placed in the +2 position on the template.
As template-based methods are most efficient for protein 3D structure modeling, combining multiple threading alignment information should increase the accuracy and reliability of computational domain predictions.
The cross-linking was most efficient if the single-stranded region of the template strand consisted of a run of dGuo's, indicating that dGuo was involved in the cross-link.
Instead, template-based modeling is the most efficient technique.
As expected, dGMP incorporation was most efficient when the primer terminus contained dC opposite dG in the template.
Optimal nanostructuring and surface area values are obtained for BiVO4 nanoplatelets synthesized at pH 4. Screening of phosphorus-containing templates brought forward dimethyl methylphosphonate (DMMP) as the most efficient surfactant to fine-tune the synthesis of hierarchically structured BiVO4 architectures.
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