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Briefly, optimal regions for designing esiRNAs were chosen using the Deqor design algorithm (Henschel et al, 2004) in order to fulfill two criteria: to obtain the most efficient silencing trigger in terms of silencing efficiency, and to get lowest chances to cross-silence other genes.
However, HD Ad HBV pri-miR-31/5-8-9 administration resulted in the most efficient silencing of HBsAg expression.
A time-course evaluation revealed that CD49e-siRNA-triggeredCD49e-siRNA-triggeredCD49e-siRNA-triggerednsientransientst efficient silencing at 72h post-transfection.
Line AS-h 3, which is the one showing the highest increase in total carotenoids (Table 2), also shows the most efficient silencing of endogenous CHY2.
Line AS-e 2, which is the one showing the highest increase in β-β-xanthophylls (Table 1), also shows the most efficient silencing of endogenous LCY-e.
The most efficient silencing was achieved with shA19e22, which downregulated the levels of expression of ADAMTS19 to less than 30%% of untreated levels in SW480 (Additional file 1: Figure S5).
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We first evaluated the silencing efficiencies of SOD1 siRNAs and selected the two most efficient silencers (si SOD1-2 and -3).
Since shTrio5 was most efficient in silencing Trio expression, this construct was used in subsequent experiments.
Co-expression of viral silencing suppressor proteins can increase and prolong protein expression, but highly efficient silencing suppressors may stress plant tissue and be detrimental to protein yields.
Our results show that the most efficient gene silencing was achieved using fully de-N-acetylated chitosans with intermediate chain lengths (DPn 100 300).
Among several exon10 specific siRNAs, we selected the one by which the most efficient gene silencing could be obtained in HepG2 liver cells.
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