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The most efficient peptide substrates in some cases overlap and in other cases differ between the two enzymes tested, and a range of kcat values was obtained.
The behavior is different, when the most efficient peptide A6D is loaded (Figure 7B, O).
CoilB, which was designed to block the last 15 aa of the coiled-coil structure in the C-terminus of EspA, was the most efficient peptide in inhibiting EspA polymerization, while CoilA, which was designed to block the first 15 aa of the same coiled-coil structure, was less efficient than CoilB.
PTHrP produced the highest stimulation of PTH1R (p < 0.05) and together with PTH-L was the most efficient peptide (Table 2).
The same group tested active immunisation with different Aβ species in young lemur primates in order to evaluate the antibody response and choose the most efficient peptide and adjuvant for further studies in old lemurs [ 82].
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Interestingly, the authors screened a series of carbonic acid derivatives as S-acylating agents, among which, phenylthiochloroformate 2 proved to be the most efficient for peptide cleavage.
The most efficient albumin-derived peptide (peptide #6), was selected for in vivo testing.
The most common method is to affinity purify the serum over a column with the peptide immobilized on beads, a procedure that is most efficient when the peptide is conjugated to a protein, such as keyhole limpet hemocyanin (KLH; Daniel Louvard, personal communication).
We evaluated the influence of ALA, phenylalanyl derivative 1 (the most efficient of the peptide derivatives) and Ac-Ala-Ala-Ala-Ala-OH, which is a substrate for ACPH (Krishna and Wold, 1992; Sokolik et al, 1994).
In particular, the peptide corresponding to ARID1A is the most efficient non-fluorophore conjugated peptide substrate of Zn-HDAC8 discovered to date (kcat/ KM = 740 M–1 s–1).
Four molecular classes were investigated in this study, with relative gas-phase densities observed as follows, from least to most efficient packing: TAA salts, lipids, peptides, and carbohydrates.
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