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Global chromosome analyses like 2D-gels usually reveal the most efficient origins [41], [42].
Using this method the location and time of activation of the 332 most efficient origins were determined.
Alternatively, it is possible that the origin activity of NUMTs has not been detected in S. pombe because the analysis revealed only the most efficient origins, and NUMTs may function as late or weak origins.
A previous investigation revealed the detailed topography (i.e. the location and the time of activation) of the most efficient origins in S. cerevisiae chromosomes based on oligonucleotide microarrays [42].
Therefore, mutants that determine adverse conditions for replication initiation or factors that limit the most efficient origins are used to identify other origins or determine the conditions and the hierarchy of usage [26], [44]).
It is intriguing that most efficient origins appeared to fire late in S-phase in cells released after HU block (Figure 5B and 5C), given that the method applied was known to favor early-firing origins [2], Rad3p-Cds1p mediated checkpoint is known to restore stalled forks during HU-induced DNA replication block [9], [15], [19].
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To test if disruption of an origin of replication can increase the local mutation rate in an early-replicating/low mutation rate region, the earliest and most efficient origin, ARS607, was deleted in strain GL·36, where the URA3 gene is located 3 kb away from the origin.
As control, we inserted well-characterized ARS1 [33] and ARS121, one of the most efficient known origins [27], [34], and a variety of other sequences (random genic and intergenic yeast sequences, see Figure 1C and Table S2).
The evaluated DMU (E _{3}) is closer to the coordinate origin than the most efficient production frontier, and then the efficiency of DMU (E _{3}) is higher than that of every sample DMU from the constraint condition.
In this letter, we review the development of polymeric solar cells from its origin to the most efficient devices published to date.
For Most Efficient and Fastest Service the Line Must . . .
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