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Type 1 SSRs (i.e. repeat sequences with a length greater than 20 bp), are considered to be hypervariable and the most efficient loci for use as molecular markers [ 64].
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Although reliance on nrITS as the sole source of phylogenetic evidence has come under criticism because of certain features of its evolution [ 9], it remains the most efficient locus for generating species-level phylogenetic inferences in most plant groups.
In general, the level of interspecific SSR sequence conservation, even amongst more closely related species within a single clade, was low and the method may not be the most efficient means of identifying novel SSR loci.
Digestion of the PCR products allows for the discrimination of the A, B and O alleles and is the easiest and most efficient way of genotyping at the ABO locus as well as determination of LOH [8], [38] [40].
This region from the 18S rDNA is considered as the most variable locus of this complex, and therefore, it is the most efficient species marker for fungi (Nilsson et al. 2008), making it the most appropriate region of the fungal DNA for the development of a molecular screening tool.
A high degree of reliability was obtained for individual-breed assignment from the 18 loci by using different approaches among which the Bayesian method provided to be the most efficient, with an accuracy for nine microsatellites of over 99%.
Comparative multi-locus analyses based on both nuclear and mitochondrial genetic markers are probably the most efficient and informative approach to discerning the relative role of historical events and life-history traits in shaping genetic heterogeneity.
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