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To identify the most efficient genomic DNA extraction method, four commercial kits and two homemade methods were examined using samples containing about 70,000 cells.
The potential extra gain for the breeding goal produced by the most efficient genomic scheme (GE_ref) compared to the most efficient pBLUP breeding scheme (BL_90%) depended on the relative economic weights of the traits in the breeding goal.
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Cryopreservation is the most efficient means of preserving genomic material [ 4, 42, 57, 61, 62].
With respect to the major QTL, the most efficient selection schemes were those combining genomic selection with gene-assisted selection for the target QTL.
In order to assess in vivo the functionality of the most efficient miR SOD1 sequence, 8.4E9 viral genomic copies (vg) of AAV6-cmv RFP miR SOD1 were injected in the triceps surAAV6-cmv RFP miR3ASOD1 mice.
Implementing genomic evaluations was the most efficient approach when major expenditure was possible, whereas increasing phenotypes was preferable when limited resources were available.
One of the most efficient and widely used approaches for comparing genomic variation within species uses single nucleotide polymorphism (SNP) panels [ 1, 2].
Since even the most efficient WGA platform could not provide complete genomic coverage, genotype imputation of the SNP data obtained from the Illumina platform is necessary in order to complete the genotypic information required for precise estimation of breeding value.
Finally, WU BLAST (Lopez et al., 2003) was employed to define more precisely short or 'strange' genomic regions, as WU BLAST has proven most efficient when transcript composition is unknown (Elizabeth Cha and Rouchka, 2005).
Developing ways of storing and sharing genomic data will also be crucial, to make the most efficient use of these resources for conservation.
Successful sample production involves maximizing yield at both steps (gDNA extraction and WGA), since incomplete release of gDNA from the cells necessarily leads to low genomic coverage at the amplification step, even with the most efficient DNA amplification system.
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