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TALENs (transcription activator-like effector nucleases), consisting of a custom TALE DNA binding domain fused to a nonspecific FokI cleavage domain, are one of the most efficient genome engineering methods developed to date.
RIP is likely the most efficient genome defense mechanism against TEs and has been experimentally demonstrated to be currently active in L. maculans [ 47].
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Both MPSS and microarrays were the most efficient genome-wide transcript profiling methods and they correlated well.
Rearranging the genome to select the most efficient gene combination has been described as a common way to adapt quickly to extreme environments [ 34].
After evaluation, we chose KpnI as the most efficient enzyme for the ostrich genome for use in subsequent experiments.> All work done in this project followed the guidelines and protocols for research on animals and had the necessary permits and authorization.
Less than 5 years ago the CRISPR/Cas nuclease was first introduced into eukaryotes, shortly becoming the most efficient and widely used tool for genome engineering.
Resequencing is the most efficient approach for a large scale, genome-wide SNP discovery.
However, in case of complex genomes, even the most efficient methods are not able to correct all the errors from the long reads (see Results and discussion section for more details).
Recently, the genome sequencing technology has emerged as one of the most efficient tools that can provide whole information of a genome in a small period of time.
Therefore, plastid genome hybridization enrichment will be most efficient in terms of time and money for projects that involve sequencing of hundreds of plastid genomes.
Lentiviral delivery is currently among the most efficient methods but it integrates transgenes into the genome, which may affect the behavior of the iPSC if integration occurs into an important locus.
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