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C2, the most efficient DNA intercalator, showed the strongest DNA photocleaving activity via superoxide anion produced under UV light at 360 nm.
B4 (p-CH3), the most efficient DNA photocleaver, showed detectable DNA cleavage at 0.5 μM and total cleavage from form I to 100% form II at 50 μM.
A2 was the most efficient DNA photocleaver, which had converted supercoiled DNA from form I to form II at <1 μM.
B2, the most efficient DNA photocleaver, also exhibited the highest cytotoxicity with the IC50 of 2.53 and 0.11 μM against cell lines of A549 and P388, respectively.
Another 11 cycles were performed with the reaction time progressively reduced from 4 h to 6 s for the purpose of enriching the most efficient DNA sequences (see Materials and Methods for the reaction time allowed for each cycle).
By far the most efficient DNA deposition is observed on the Zeonex surface, followed by PMMA and CYTOP, where barely any DNA is deposited at all (Supporting Information, Figure 1).
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The most efficient target DNA capture was observed using short PCR amplicons (69 bp).
However, methylated CGs were decreased to 23.75%, 21.5% and 12.5% when DNMT1,DNMT1/DNMT3A and DNMT1/DNMT3B were subjected to double knock down with 10 selected clones (Fig. 1E), indicating that knockdown of DNMT1 and DNMT3B together was the most efficient for DNA demethylation.
To identify the most efficient genomic DNA extraction method, four commercial kits and two homemade methods were examined using samples containing about 70,000 cells.
PLL of the smallest length chosen here was found be the most efficient in condensing DNA at low DNA concentrations.
N-Phenacylthiazolium bromide extraction, a method frequently used for extraction of ancient DNA, proved to be most efficient to extract DNA from wood of G. bancanus (Asif and Cannon 2005).
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