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The most effective genes with the strongest antimicrobial activity, BmcecB6, BmcecD and Bmmor, had the highest level of induction, suggesting that high induction is a hallmark of the most effective AMP genes in the antimicrobial response.
Besides, to select the most effective genes, "timeROC" package was utilized.
Based on the AUC, three genes were diagnosed as the most effective genes on OPL patients' survival.
Finally, five most effective genes (8126931, 8120206, 7971191, 8161169, and 7897663) were selected using area under ROC curve by means of timeROC package.
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Sequence variability among paralogous or hom e)ologous allelic loci has to be considered, since the most effective gene copy should be selected in order to avoid non-additive effects [ 42].
The most effective gene delivery was obtained with nonpathogenic adeno-associated viruses (reviewed in Ref. 100).
Utilization of siRNAs is one of the most effective gene silencing methods and is a promising new approach in the analysis of gene function and gene therapy [ 22- 25].
We again reasoned that the most effective gene signature would yield the best opposite-quadrant separation of LPI and HPI samples.
While viral vectors are the most effective gene delivery systems and have been used in several clinical trials, their intrinsic immunogenicity, potential side effects (e.g., insertional mutagenesis), limited nucleic acid packaging capacity, and difficulties of mass production will restrict their application in gene therapy.
The most effective 40 genes caused a mortality of 50 100 % at day eight post injection using the lowest concentrated dsRNA (Additional file 1: Table S2, Additional file 2: Figure S1 A).
We focused on the 11 most effective target genes, which were marked by mortality of 100%% on day eight and at least 80%% on day six post injection (Fig. 1b– m).
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