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Exact(2)
Since AH.FC.09 and AH.FEr.09 were the most effective fractions, we further assessed its effects on cell proliferation, metabolism and death.
To assess the effect of propolis on cell proliferation, HCT-15 cells were treated with IC50 concentrations, as well as two higher concentrations of the most effective fractions, AH.FC.09 and AH.FEr.09.
Similar(58)
When studied in acetic acid induced writhing test, the extract/fractions evoked significant antinociceptive effect in which chloroform was the most effective fraction followed by ethyl acetate.
The most effective fraction F1 was further purified and subjected to 1H, 13C-NMR and mass spectrometry analysis and the compound was identified as icariside E4.
E. coli was found to be the most resistant bacteria with the MIC of 12.5 mg/ml for the most effective fraction and 50 mg/ml for the least effective fraction.
Mao et al. [ 28] concluded that the lower was the molecular weight of the yak casein hydrolysate fraction, the higher was its ACE-I inhibitory activity, and the molecular weight of the most effective fraction was below 6 kDa (85.4%).
To detect the most effective fraction, the antiproliferation efficacy of four fractions (namely derivatives by adding EAC to n-BuOH, petroleum ether, EtOAc and water until dissolve fully) against five cancer cell lines were screened by MTT assay.
The order of potency in SRB assay was found to be similar as MTT assay with NJDE being the most effective fraction in MDA-MB-231 cells with an IC50 of 23.80 ± 0.66 μg/mL (Table 5).> -wrap-foot> All the values are expressed as mean ± SEM (n = 3).
The HMG-CoA reductase assay kit from Sigma-Aldrich (St . Louis MO, USA) with the catalytic domain of the human enzyme (recombinant GST fusion protein expressed in E. coli) was used, under conditions recommended by the manufacturer, to identify the most effective fraction of plant extract.
Minimal inhibitory concentrations (MICs) of the most effective cranberry fraction (FC111) were determined against multiple S. aureus strains and drug interactions with β-lactam antibiotics were also evaluated.
The exceptional fraction, Fraction P, which contains most of the polycyclic aromatic hydrocarbons of smoke and is the most effective of the fractions tested in producing tumours in mouse skin, gave rise to 4 squamous tumours of doubtful malignancy and one metastasizing squanmous carcinoma among 3 groups of 18 animals exposed at 3 different dose levels.
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