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The most drastic influence on the enzyme activity was demonstrated by the mutants D382A, V209D, W377A and K487A resulting in a sharply declined activity (a reduction by 85.9%, 87.9%, 93.9% and 96.3% in the activity and 7.1 fold, 8.39 fold, 17.3 fold and 27.7 fold in the Kcat values, respectively) as compared to the activity of the native protein.
While surface chemistry appeared to have the most drastic influence on AuNPs' behavior in our biological test system, the data could also be interpreted to suggest that AuNP behavior is modulated by core size.
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