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In addition, most downstream analysis tools for gene lists enable analyses of only one ontology abstraction level [21].
This text file format is compatible with most downstream analysis tools such as BioConductor, Cluster or ProfileSoftware [ 46- 48].
Hence PE/PPE genes were excluded from most downstream analysis including SNP calling and gene content analysis.
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Most common downstream analysis tools will be able to natively support the sorts of comparisons necessary for comparing between species with minimal effort or changes to existing workflows.
ASSIsT thus proved to be an effective tool for genotyping studies as it allows to easily filter informative and well-performing SNP and to recover potentially useful SNPs from indels or regions of high-sequence divergence, feeding them directly to the most common downstream analysis tools through its easy interface.
Among the wAnaITG genomes the majority of the polymorphic sites with a single nucleotide variant or small-sized INDEL was PSHET (table 2) and filtered out for most of our downstream analysis.
Those recent methods have been found to produce the most reliable alignments for downstream analysis using the PAML software [ 57, 58].
There are also other types of OTU clustering and picking strategies being developed 138– 141; the most appropriate choice for the downstream analysis will depend on the type of data and the user.
Also, it seems a bit inconsistent to say that the ENCODE quality control standards are used for the replicates if later only the replicate which gives the most peaks is used for downstream analysis.
Probably the most challenging area currently, is the downstream analysis of data, mainly because of the increased data set size.
Because these very common side effects may be less likely to reflect target-mediated effects, and in order to prevent them from overwhelming and biasing the downstream analysis, we excluded 27 of the most common side effects (defined as observed with at least 10% of drugs; Supplementary Table 5), after which 1179 side effect types remained.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com