The downregulated enrichment or DRE ranks from most downregulated to the least downregulated.
Only the top 50 (25 most upregulated and 25 most downregulated) were selected at this stage.
Hypocretin was the most downregulated gene in human narcolepsy brains.
We noted that the most downregulated transcripts were the immediate early response genes, early growth response 1 (Egr1) (−2.5 fold, the most downregulated transcript in the entire array) and FBJ osteoscarcoma oncogene (Fos) (−1.9 fold, the second most downregulated transcript) (Fig. 5C).
Signal transducer activity was the most downregulated molecular function (F) term, and cell communication was the most downregulated biological process (P) term.
Ing2 was the most downregulated gene (Table S1), confirming the validity of the experimental system.
Moreover, GO analysis showed that protein biosynthesis was the most downregulated biological process term.
Among the most downregulated genes by CENP-A silencing are SKP2, RAD51, and CCNG1.
Membrane and related terms were the most downregulated cellular components (C) (Table S4).
The most downregulated gene in vivo was lactate dehydrogenase.
