The downregulated enrichment or DRE ranks from most downregulated to the least downregulated.
Several of the most downregulated genes (for example, ST3GAL6, CT45, LPXN, RHOU) have been implicated with cellular adhesion and motility, whereas many upregulated genes are associated with activation of PI3K/AKT/mTOR signaling (for example, LGALS1, EIF4E2, MAPKAP1, SEMA4D) and cell proliferation (for example, BCL11A, RAB31).
A higher number of downregulated cellular genes or a higher degree of downregulation was observed at the RPF levels at 3.5 hpi and 6.25 hpi.
Remarkably, five of the most downregulated miRNAs, including miR-34b-5p, miR-34c-5p, miR-200b-3p, miR-200b-3p, and miR-200c-3p, were linked to the NOTCH pathway, although in different cellular contexts [ 3, 4, 26, 31, 70].
Only the top 50 (25 most upregulated and 25 most downregulated) were selected at this stage.
Hypocretin was the most downregulated gene in human narcolepsy brains.
We noted that the most downregulated transcripts were the immediate early response genes, early growth response 1 (Egr1) (−2.5 fold, the most downregulated transcript in the entire array) and FBJ osteoscarcoma oncogene (Fos) (−1.9 fold, the second most downregulated transcript) (Fig. 5C).
Signal transducer activity was the most downregulated molecular function (F) term, and cell communication was the most downregulated biological process (P) term.
Ing2 was the most downregulated gene (Table S1), confirming the validity of the experimental system.
Moreover, GO analysis showed that protein biosynthesis was the most downregulated biological process term.
