Indeed, the NanA tree can be subdivided in five main lineages (named I, II, III, IV and V) (Fig. 3b), with Lineage I as the most divergent clustering, encompassing Gram-negative genera, Actinobacillus, Haemophilus and Pasteurella, Fusobacteria, Gram-positive genera, Clostridium, Lactobacillus, Staphylococcus, and Mycoplasma (Fig. 3b).
The fHbp locus was the most divergent between Cluster 1 and Cluster 2. The most common fHbp allele in Cluster 2 strains was allele 22 belonging to variant 2/family A present in 80.2% (117/145).
The most divergent group (Cluster C; Fig. 3) included mature fruits from Ved and Dul, along with Ved fruits at 35 DAP, which coincided with the peak in ethylene production (Fig. 1b).
The I2 locus represents a most divergent resistance gene cluster in Solanum.
We used an automatic filter to remove the most divergent sequences inside each cluster.
However, even if the proportion of outliers in the most divergent populations within the remaining cluster (i.e. northeast Arctic cod and Baltic Sea cod) might be mildly underestimated, we expect ascertainment bias to play a relatively minor role on regional and local scales based on theoretical expectations and our own evaluation of data.
Generally, in highly structured data sets, as K is increased the most divergent groups separate into distinct clusters first [ 18, 23].
Secondly, the clustering identifies the most divergent breed samples in a convenient graphical output.
Of the three strains examined in this study, the cluster 3 strain is the most divergent.
We investigated the similarity in the global gene expression profiles among the three placental compartments and 16 HBM2.0 tissues using average linkage hierarchical clustering of the top 1,000 most divergent genes.
