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Next, we focused our analysis on miR-10, as this was the miRNA with the most distinct expression profile in the RNA-seq experiment, being highly expressed in HB but absent from FB and MB patterned human neural progenitors.
More broadly, it's about how one culture reached out to another in order to find the most distinct expression of itself.
Following ANOVA analysis, P<0.05, we selected for a minimum five fold difference in expression level in any pairwise sample type comparison, thereby identifying 786 probesets with the most distinct expression patterns (Fig. 1)(Supplementary Data S1).
The ovary is more similar to the somatic tissues, whereas the testis harbors the most distinct expression pattern.
This was further emphasized in a heat map based on the relative miRNA expression in the various prostate tissue specimens (Additional file 1: Figure S1), whereupon the most distinct expression differences occurred between Tu and BPH tissue samples.
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Compared with the total population average, subjects with the most distinct gene expression profiles live in Olen (expressions well above the population average) as well as in the fruit cultivation region and in Gent (both with expressions well below the population average).
The embryonic endothelial cells showed the most distinct gene expression signature, while the adult glomerular endothelial cells from normal and db/db mice were most similar.
23 Among the individual signatures, the BRAF oncogenic-specific signatures showed the most distinct gene expression pattern, and had the best performance with an overall accuracy of 91%.
Principal component analysis indicated that the gene expression profiles of colon and rectal carcinomas are very much alike, similarly as ovarian and uterine tumours, whereas glioblastoma has the most distinct gene expression pattern.
We showed that these genes have weak basal expression throughout rice development, and most have distinct expression patterns mainly in the secondary meristems as revealed by mRNA in situ hybridization analyses.
The most distinct lamina-specific expression patterns were noted for Map1b (layers V VI and II), N200 (layers V VI) and Tbr1 (layers IV VI) (Fig. 1b d).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com