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The resulting alignment contained 106 haplotypes with a pairwise uncorrected sequence divergence of 1.6% between the most distant haplotypes.
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Total genetic distance, as well as total length of sequence (bp) between the most distant SNPs in haplotype blocks, was calculated to estimate the observed and expected recombination rate within haplotype block regions.
The most distant Tef-rht1-1 haplotypes are 1 and 10.
Therefore, we sequenced an additional control region fragment using primers L16462 and H222 [ 18] for 42 wolves carrying each of the 22 European haplotypes detected in Ref. [ 13] (w1-w22, see Table S1 in Additional file 1), selecting two individuals representing most distant geographic locations of each haplotype, except for haplotypes w18 and w20, each detected in one individual only.
The algorithm first selects the haplotype pair that is phylogenetically most distant (i.e., largest pairwise branch length) and adds both haplotypes to X. T X now consists of a single pair of branches.
Sequentially, the haplotype that is the most distant from T X is placed into X, updating T X with each inclusion.
Haplotypes 1 and 3 are the most distant for Tef-sd1-1 with 13 nucleotide differences (99.7% identity) in a 4.6-kb region.
For example, a few of the haplotypes from the Northern-lowland Kanlow variety were the most distant from the Southern-lowland populations.
For Tef-rht1-2, haplotypes 1 and 4 are 98.83% identical and are the most distant (27 polymorphic sites in a 2.3-kb region).
This most distant galaxy is therefore also the youngest known.
Quasars are among the most distant and luminous objects known.
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