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The most dissimilar sequences affiliated with marine alveolates (e.g. 80.6% similarity), whereas some were identical to GenBank entries (mostly prasinophytes and the haptophyte Chrysochromulina) and many were >99% similar to GenBank sequences.
In single linkage, it is the similarity between the two most similar sequences; in complete linkage, between the two most dissimilar sequences; and in average linkage, the average of all pairwise similarities.
Similar(58)
The RT-PCR efficiencies and lower limits of detection of the quantification assays were only marginally affected when tested on the most dissimilar target sequences found in the GenBank database.
Therefore, the most dissimilar region with other GM sequences was selected for primer design in order to avoid cross-reactivity with non-target sequences.
Comparison of Rotarix® vaccine strain gene sequences to wild-type G1P[8] Wa strain sequences found that the Rotarix® NSP2 gene was most dissimilar with 91% identity followed by 93% identity between Rotarix® and Wa NSP1 gene sequences (Table 1).
Therefore, we designed specific primers targeting distinctive regions of these genes using a Clustal-based sequence alignment, as a starting point to select the most dissimilar regions, and Primer-Blast.
Most dissimilar regions were found in the C- and N-termini, and higher conservation between sequences was seen in the territories that were occupied more recently by humans (North America and South America).
The most dissimilar members of the wheat caleosins gene family were Clo10 and Clo4 which had 37% amino acid sequence identity and 57% similarity within the conserved domain.
Crataegus monogyna is most dissimilar, and in this respect these limited metabolomics results parallel the phylogenetic relationships determined from both chloroplast DNA sequence data (Fig. 1) and nuclear DNA (Lo et al. 2007, 2009; Zarrei et al. 2014).
The conformer most dissimilar to the first two dissimilar conformers is chosen as the third diverse conformer.
Unsurprisingly, the most dissimilar countries were Russia and Japan (at 9%) and the USA and Russia (at 11%).
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