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This is likely due to our finding that, before any differentiation period, primary sphere colonies expressed mRNA for most differentiated cell markers.
Whereas the nuclear envelope and nucleolar periphery are sites of chromatin compaction in most differentiated cell types, these regions in one-cell embryo nuclei did not display significant chromatin accumulation (Figure 1C and Figure 2A D).
We note that the most highly "plastic" cell type included in this study, i.e. melanoblasts, had the largest number of unique sequence tags as well as the most known and predicted miRNAs, whereas the most differentiated cell type (melanocytes) had the least (Tables S1A and S1B, and Table 2).
p53 is an anti-proliferative protein and highly abundant in mESCs (Supplementary Figure S1A), a cell type that proliferates faster than most differentiated cell lines (Supplementary Figure S1B).
Ciliopathies are a recently defined and rapidly growing group of human disorders caused by dysfunction of primary cilia which are microtubule-based organelles present on the surface of most differentiated cell types (1– 3).
Notably, miR-143/-145 and PAI-1 levels in the three most differentiated cell lines are inversely represented with MGH-U4, having the highest PAI-1 and the lowest miR-143/-145 expression.
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By the end of immune adaptation in early ontogeny, the MDC are proposed to encounter the most differentiated cells in a specific tissue, and prevent them from differentiating beyond the encoded state during adulthood by the so called "stop effect".
In higher plants, however, most differentiated cells are able to transdifferentiate, i.e. start a new differentiation pathway.
Most differentiated cells, including cardiac myocytes, express multiple connexins.
The intestinal epithelium undergoes rapid turnover, and most differentiated cells are replaced with newer ones in less than a week (Barker, 2014).
iPSC-Heps can be directed to differentiate into HLCs by mimicking embryonic and fetal liver development (Hannan et al., 2013; Kajiwara et al., 2012; Takebe et al., 2013), but most differentiated cells co-express AFP and ALB, suggesting incomplete cell maturation.
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