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It was fitting: Arjen Robben, the Dutch wing who had been roundly criticized for struggling in the biggest moments, starred in the most critical sequence of all, scoring the decisive goal for Bayern in the 89th minute and lifting the Bavarians to a 2-1 vintory in front of a crowd announced at 86,298.
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These structural observations are consistent with RNA binding experiments with all possible single substitutions in 3′ end nucleotides 1 11, which show that nucleotides 6 8 are the most critical for sequence specific binding.
These substitutions remove most of the critical sequences identified by ChIP-Seq assays (Harrison et al., 2011; Nien et al., 2011; Perry et al., 2011).
Tags mapped to a unique sequence are the most critical subset of the DGE libraries as they can explicitly identify a transcript.
Tags mapped to a unique sequence are the most critical subset in DGE libraries, as they can explicitly identify a transcript.
Tags mapped to a unique sequence are the most critical subset of the DGE libraries because they can unambiguously identify a transcript.
We also considered the seed sequence (positions 2 7) separately from other parts of the mature sequence because the seed sequence is known to be most critical for target recognition (see Bartel 2009 for review).
Kinase specificity is often achieved by recognition of amino acids surrounding the site of phosphorylation, therefore we attempted to identify sequences that are most critical for LRRK2's optimal activity.
A second advantage, and perhaps the most critical, is that MAD appears stable when sequence divergence is unrealistically high (e.g. tree length scaled by a factor of 2; Figure 1).
Primers and probe sequence design are among the most critical factors in real-time polymerase chain reaction (PCR) assay optimization.
Primer and probe sequence designs are among the most critical input factors in real-time polymerase chain reaction (PCR) assay optimization.
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