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While most control cells had reached the subsequent G1 phase by 14 hours, PIASγ siRNA-treated cells accumulated in mitosis (∼35%) and the mitotic index remained high up to 24 hours after the release of the thymidine block (Fig. 1I,J and data not shown).

At this time point, most control cells were spindle shaped and had a mean length of about 110 μm (109.5 ± 17.2 μm; Figures 1D and 1E).

Indeed, most control cells migrated upstream a concentration gradient of EGF, but NAV3-depleted cells failed reading the gradient (Fig 5D).

The data are also shown as acceleration vector diagrams (Fig. 2G), highlighting that most control cells were repelled from one another after collision, whereas RhoA knockdown cells did not switch their direction of migration after contact.

Six hours after radiation, most control cells had no dramatic changes in γ-H2AX staining, while the foci in half of the VCP-knockdown cells had disappeared (P<0.01).

The data are also shown as vector diagrams (Fig. 4F), highlighting that most control cells are repelled by cell cell contact, whereas, like RhoA knockdown cells, Vav2 knockdown PC-3 cells continued migrating in the same direction regardless of whether they made contact with another PC-3 cell or not.

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Similar(52)

Most control T cells had a migratory phenotype, characterized by a clear polarized morphology (Figure 2E, F, Movie S3), with a single lamella containing an F-actin-rich lamellipodium and highly dynamic membrane ruffles, and a uropod frequently protruding out of the plane of migration.

While most of control cells re-entered a new division cycle at 16 h after release from G1/S, a significant proportion (48%) of hCdc14B-depleted cells was enriched in metaphase (Fig. 3B and Table 1), suggesting that hCdc14B activity is required for successful completion of mitosis.

Whilst in most of control cells the EB1 comets displayed θ angles within 20° of this 180° alignment, those in hMsd1-depleted cells showed a much broader range of angles (Fig  2H and I).

In line with this observation, SOCS3-transduced cells also lost viability in response to IL7, whereas most control-transduced cells maintained their viability (Fig. 5E).

This observation most certainly indicates that most of the control cells are retained in the quiescent (G0) state which is consistent with their inability to grow under these growth conditions.

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